An Effective Growth Medium for Probiotics for Strict Vegetarians

Development of MMRS (modified MRS Agar) culture medium; the absolute vegetarian culture medium containing plant seed powder in place of beef extract for the growth of probiotics. Vegetarian probiotic foods by definition must be free from all animal-derived ingredients. This research provides an alternative efficacious, cost effective vegetal culture medium. The culture medium developed in present invention will provide a pure vegetarian way to achieve pure vegetarian products for strictly vegetarian human population. In countries like India people of certain religion are seriously concerned about such issues. Present study investigated the growth of a pure culture strain of Lactobacillus lactis, Lactobacillus casei, Lactobacillus plantarum, Bifidobacterium strain 231 and Bifidobacterium strain 234 provided by National Dairy Research Institute (NDRI), Karnal (Haryana, India), in MRS and MMRS. The developed culture medium is better than standard MRS and this will be a great relief for such communities.